Next-Generation Sequencing Informatics
Below is a table with informatics and IT statistics for the major next-generation/massively parallel sequencing platforms. The information in the table is approximate and should only be used for general, informational purposes.
Next-Generation Sequencing Statistics
Notes:
- Units: B – bytes, b – bases
- PA is primary analysis (includes image feature extraction and base calling)
- PA CPU is calculated as the wall clock multiplied by the number of CPU cores
- ABI SOLiD data, except rate, are representative of a single slide
- ABI SOLiD and Illumina GA IIx primary analysis is done on instrument
- 454 paired-end reads vary in length depending on location of internal adapter
- SRA is the size of the files (SFF, SRF, or FASTQ) that are submitted to the NCBI Short Read Archive
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| Vendor: | Roche | Illumina | ABI | Technology: | 454 | Solexa GA | SOLiD | Platform: | GS20 | FLX | Ti | I | II | IIx | 1 | 2 | 3 | Reads: (M) | 0.5 | 0.5 | 1.25 | 28 | 100 | 250 | 40 | 115 | 320 | Fragment | Read length: | 100 | 200 | 400 | 35 | 50 | 100 | 25 | 35 | 50 | Run time: (d) | 0.25 | 0.3 | 0.4 | 3 | 3 | 5 | 6 | 5 | 8 | Yield: (Gb) | 0.05 | 0.1 | 0.5 | 1 | 5 | 25 | 1 | 4 | 16 | Rate: (Gb/d) | 0.2 | 0.33 | 1.25 | 0.33 | 1.67 | 5 | 0.34 | 1.6 | 4 | Images: (TB) | 0.01 | 0.01 | 0.03 | 0.5 | 1.1 | 2.8 | 1.8 | 2.5 | 1.9 | PA Disk: (GB) | 3 | 3 | 15 | 175 | 300 | 300 | 300 | 750 | 1200 | PA CPU: (hr) | 10 | 140 | 220 | 100 | 70 | NA | NA | NA | NA | SRA: (GB) | 0.5 | 1 | 4 | 30 | 50 | 2.5 | 100 | 140 | 600 | Paired-end | Read length: | 200 | 400 | 2×35 | 2×50 | 2×100 | 2×25 | 2×35 | 2×50 | Insert: (kb) | 3.5 | 3.5 | 0.2 | 0.2 | 0.2 | 3 | 3 | 2 | Run time: (d) | 0.3 | 0.4 | 6 | 10 | 10 | 12 | 10 | 16 | Yield: (Gb) | 0.1 | 0.5 | 2 | 9 | 50 | 2 | 8 | 32 | Rate: (Gb/d) | 0.33 | 1.25 | 0.33 | 1.67 | 5 | 0.34 | 1.6 | 4 | Images: (TB) | 0.01 | 0.03 | 1 | 2.2 | 5.6 | 3.6 | 5 | 3.8 | PA Disk: (GB) | 3 | 15 | 350 | 500 | 550 | 600 | 1500 | 2400 | PA CPU: (hr) | 140 | 220 | 160 | 120 | NA | NA | NA | NA | SRA: (GB) | 1 | 4 | 60 | 100 | 3.5 | 200 | 280 | 1200 | ||||||||||||||||||||||||||||||||||||||||
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December 5th, 2008 at 7:35 am
[...] estimations of our disk space requirements in the next couple of years. I came across this table (Next-Generation Sequencing Informatics Statistics) and thought it would be [...]
Next Generation Sequencing » Overview of NGS platforms:
December 5th, 2008 at 10:03 am
[...] David Dooling over at PolITiGenomics has compiled a nice table with some important metrics of the three most prevalent NGS platforms – go check it out! [...]
Next-Generation Sequencing Statistics « ScienceRoll:
December 8th, 2008 at 3:52 pm
[...] trackback Have you ever wondered about the typical run time of a next-generation genome sequencer? PolITiGenomics has the [...]
:
December 16th, 2008 at 3:26 pm
[...] PolITiGenomics Next-Generation Sequencing Informatics – Great overview of the current technologies.tags: science genomics bioinformatics [...]
Managing and Analyzing Next-Generation Sequence Data « LabLynx LiMS Journal:
July 11th, 2009 at 2:54 pm
[...] Dooling D2008 Dec 4. Next Generation Sequencing Informatics Table. https://politigenomics.com/next-generation-sequencing-informatics . [...]
Pathogens: Genes and Genomes » Do you store your image files?:
October 6th, 2009 at 12:04 am
[...] not sure that the storage cost argument is right. Referencing David Dooling’s just-updated next-generation informatics table you are looking at 2.8 terabytes of image data per GA2 run. Phil Green suggests these can be [...]
Next Generation Sequencing » NGS platforms overview:
November 24th, 2009 at 7:36 am
[...] can find the original table here. As the original compilation, this work is openly available through an Attribution-Share Alike 3.0 [...]
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February 16th, 2010 at 5:40 pm
[...] somewhat dated comparison of next-generation sequencing platforms on another blog, PolITiGenomics (which also examines newer platforms when they are [...]
Managing and Analyzing Next-Generation Sequence Data | LIMSblog.com:
January 19th, 2013 at 6:35 pm
[...] Dooling D2008 Dec 4. Next Generation Sequencing Informatics Table. https://politigenomics.com/next-generation-sequencing-informatics . [...]